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DronePort Rotterdam is an innovation ecosystem dedicated to the development, testing, and application of autonomous and uncrewed systems (AUS) in real-world operational environments. Strategically located at the Port of Rotterdam, we integrate drones and related technologies across the port, city, and Zuid-Holland region. Our approach emphasizes both vertical and horizontal integration: connecting air, land, water, and space to create a cohesive operational environment while fostering collaboration across sectors like maritime, logistics, emergency response, offshore, defense, public safety, and port and urban development. By aligning technological, regulatory, and infrastructural standards, DronePort Rotterdam not only addresses local needs but also establishes a scalable blueprint that other ports and regions worldwide can adapt.